Portrait
Andres Veide, PhD

Associate Professor of Bioprocess technology

Director of Undergraduate and Master Studies

 

Department of Biotechnology
Roslagstullsbacken 21

Royal Institute of Technology (KTH)
S-106 91 Stockholm
Sweden

 

Phone: +46-8-553 783 14
Fax: +46-8-553 783 23
Email: veide@kth.se

 

Teaching

Director of Undergraduate and Master Studies at the School of Biotechnology.

Examiner in the course BB101X Degree Project in Biotechnology; First Level 15.0 credits

Examiner in the course BB102X Degree Project in Engineering and Management, First Level 15.0 credits

Research interests

The research is focused on the downstream processing of proteins. In particular, we are studying different primary recovery operations, their integration upstream and downstream, and their scale-up. Techniques of particular interest have been aqueous two-phase extraction and expanded bed adsorption. To change and control solubility and partition behaviour of proteins, gene technology has been used to alter their structure and surface properties. In connection to this the effect on protein proteolysis has been studied. Physical chemical properties of cell surfaces and their surface adsorption are being studied to increase the knowledge regarding the fouling of chromatography adsorbents and filtration membranes.

Andres Veide is managing the pilot plant that was installed at the department 2002. The facility is intended for teaching, research and contract work.

Selected publications

Malmsten, M. and Veide, A. (1996) Effects of amino acid composition on protein adsorption. J. Colloid Interface Sci., 178, 160-167.

Gustavsson, K., Bergman, T., Veide, A. and Enfors, S.-O. (1997) Kinetics of in vitro complex formation between DnaK and staphylococcal protein A derivatives produced in Escherichia coli. Biotechnol. Applied Biochem., 25, 173-180.

Malmsten, M, Burns, N. and Veide, A., (1998) Electrostatic and Hydrophobic Effects of Oligopeptide Insertions on Protein Adsorption. J. Colloid Interface Sci., 204, 104-111.

Berggren, K., Veide, A., Nygren, P.-Å. and Tjerneld, F. (1999) Genetic engineering of protein-peptide fusions for control of protein partitioning in thermoseparating aqueous two-phase systems. Biotechnol. Bioeng., 62, 135-144.

Svensson, M., Berggren, K , Veide A. and Tjerneld, F. (1999) Aqueous Two-Phase Systems Containing Self-Aggregating Block Copolymers. Partitioning of Hydrophilic and Hydrophobic Biomolecules. J. Chromatography A, 839, 71-83.

Berggren, K., Tjerneld, F.and Veide, A. (2000) Peptide fusion tags with tryptophan and charged residues for control of protein partitioning in PEG-potassium phosphate aqueous two-phase systems. Bioseparation, 9, 69-80.

Bylund, F., Castan, A., Mikkola, R., Veide, A. and Larsson. G. (2000) Influence of scale up on the quality of recombinant human growth hormone. Biotechnol. Bioeng., 69, 119-128.

Bandmann, N. Collet, E., Leijen, J., Uhlén, M. Veide, A. and Nygren, P.Å. (2000) Genetic engineering of the Fusarium solani pisi lipase cutinase for enhanced partitioning in PEG-phosphate aqueous two-phase systems. J. Biotechnol., 79, 161-172.

Rozkov, A., Schweder, T., Veide, A. and Enfors, S.-O. (2000) Dynamics of proteolysis and its influence on the accumulation of intracellular recombinant proteins. Enzyme Microbial Technol., 27, 743-748.

Bandmann, N., van Alstine, J., Veide, A. and Nygren, P.-Å. (2002) Functional selection of phage displayed peptides for improved aqueous two-phase partitioning of recombinant proteins. J. Biotechnol., 93, 1-14.

Collén, A., Penttilä, M., Stålbrand, H., Tjerneld, F. and Veide, A. (2002) Partitioning of fusion proteins of endoglucanase I (Cel7B) from Trichoderma reesi in PEG-phosphate aqueous two-phase systems. J. Chromatography A, 943, 55-62.

Kepka C., Collet E., Persson J., Ståhl Å., Lagerstedt T., Tjerneld F. and Veide A. (2003) Pilot-scale extraction of an intracellular recombinant cutinase from E.coli cell homogenate using a thermoseparating aqueous two-phase system. J. Biotechnol., 103, 165-181.34.

Charoenrat T., Ketudat-Cairns M., Jahic M., Enfors S.-O. and Veide A. (2005) Recovery of recombinant beta-glucosidase by expanded bed adsorption from Pichia pastoris high-cell-density culture broth, J. Biotechnol., in press

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