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VŽronique Chotteau, Ph.D. Principal Investigator Cell Technology Group, CETEG School of Biotechnology - Div. of Bioproduction KTH - Royal Institute of Technology Albanova University Center Roslagstullsbacken 21, 1 st floor SE-106 91 Stockholm, Sweden Phone: +46 8 5537 8478 Fax: +46 8 5537 8323 e-mail: veronique.chotteau@biotech.kth.se |
Link to the Cell Technology Group, CETEG http://www.ceteg.se
Short biography
2008 - Head of the Cell Technology Group at the Division of Bioproduction, KTH
2000 - Senior Project Manager for the development of biopharmaceutical processes based on mammalian cell cultivation, Pharmacia / Biovitrum, Sweden
1997 - Head of Eucaryotic Pilot Plant at Pharmacia & Upjohn, Sweden
1996 - Laboratory engineer (eucaryotic cultivation) at Pharmacia & Upjohn, Sweden
1995 - Visiting Fellow at the Department of Biotechnology (Peter Gray, Chris Marquis), University of New South Wales, Sydney, Australia
1995 - Ph.D. in Applied Sciences (Automatic Control/Biotechnology), UniversitŽ Catholique de Louvain, Belgium
1988 - M. Sc. in Molecular Biology and Biotechnology, UniversitŽ Libre de Bruxelles, Belgium
1985 - M. Sc. in Electrical Engineering, UniversitŽ Libre de Bruxelles, Belgium
Open positions
Apply for Master Thesis in the Cell Technology Group
Research interests
The research of the Animal Cell Technology Group is focussed 1) on actual problems met in the biopharmaceutical industry in the development of animal cell cultivation processes and 2) on stem cells.
á Development of a nutrient cocktail for cultivation medium free of animal-derived components by metabolic reaction network – tool: mathematical modelling approach issued from metabolic flux analysis information
á Metabolomics of animal cellture bioprocess
á Perfusion of Chinese Hamster Ovary cell cultivation
á Development of process methods for large-scale culture of human embryonic stem cells and bio-engineered platforms that enhance cell proliferation and long-term survival of stem cell derived neurons
Services
The Cell Technology group offers a service of development of animal cell based processes suitable for industrial production of biopharmaceutical (e.g. antibody, glycoprotein) in CHO or NS0 cell-based system in preparation for clinical phase I manufacturing.
Link to CETEG Service website and Link to poster of CETEG Services
The following services are available (for track records, see poster):
¥ Development of GLP cultivation process suitable for the industrial production of glycoproteins in view of transfer to a GMP pilot plant
á expertise from more than 10 years experience in biopharmaceutical industry (at Biovitrum, previously Pharmacia) in biopharmaceutical process development management (including product like e.g. factor VIII, MAb), animal cell cultivation process development, perfusion, fed-batch, up-scaling, commercial production technical support, business development support and consulting
á development of fed-batch process
á development of perfusion process using ATF (Refine)
á development screening in CO2 incubators (shake flasks, spinners, etc.), e.g. study of feed rate and feed media
á process optimization in bioreactor scale (bioreactors (Belach): from 1 L to 20 L and GRETA six-pack mini-bioreactor screening system), e.g. study of feed rate and feed media, effect of pH, temperature, carbon dioxide partial pressure, osmolarity, etc.
á process up-scale in 20 L bioreactor (Belach), e.g. effect of aeration and gas bubbling, effect carbon dioxide partial pressure, shear stress, etc.
á study of cell metabolism, e.g. measurements of cell density, cell viability, pH, pCO2, pO2, concentrations of glucose, lactate, glutamine, ammonium by Nova BioProfile FLEX, analysis of free amino acid by HPLC, flow cytometry
á cell line production stability study
á development of seed train
á process development historical report
á process description report in view of transfer to GMP pilot plant
¥ Production of material for toxicological study in 20 L bioreactor
¥ Production of protein of interest in transient systems like HEK293 EBNA, insect cells, etc.
¥ Analytical support
á cell metabolism by Nova BioProfile FLEX (measurements of cell density, cell viability, pH, pCO2, pO2, concentrations of glucose, lactate, glutamine, ammonium)
á analysis of free amino acid by HPLC
á flow cytometry
á IgG concentration by protein A HPLC
á ELISA (e.g. HCP)
á electrophoresis
¥ The development of purification process is performed at our pilot plant
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